Author/Authors :
Pour Vahdani, Shiva Maragheh University of Medical Sciences, Maragheh , Khanzadeh, Taghi Tabriz University of Medical Sciences, Tabriz , Karimi, Fattaneh Department of Community and Mental Health Nursing - School of Nursing and Midwifery - Maragheh University of Medical Sciences, Maragheh , Jahanban Esfahlan, Aylin Department of Nursing - School of Nursing and Midwifery - Maragheh University of Medical Sciences, Maragheh , Ghavipanjeh, Somayeh Department of Psychiatric Nursing - Maragheh University of Medical Sciences, Maragheh , Ghasemi, Amin Maragheh University of Medical Sciences, Maragheh , Zadi Heydarabad, Milad Yasuj University of Medical Sciences, Yasuj , Azizi, Hakim Department of Medical Parasitology - Zabol University of Medical Sciences, Zabol , Darbin, Akbar Shahid Madani Hospital - Tabriz University of Medical Sciences, Tabriz , Khorrami, Arash Department of Basic Sciences - Maragheh University of Medical Sciences, Maragheh , Azimi, Ako Department of Basic Sciences - Maragheh University of Medical Sciences, Maragheh
Abstract :
Objectives: Acute lymphoblastic leukemia (ALL) is considered one of the common types of cancers in childhood with an incidence of up to 25%. In addition, drug resistance is a phenomenon which reduces the chances of overcoming cancer. Further, a phytoalexin combination called resveratrol can sensitize the leukemic cells to apoptotic cell death. Due to the importance of the above-mentioned issues, the present study aimed to evaluate the effect of resveratrol on BAX and BCL-2 expression levels and apoptosis induction.
Materials and Methods: CCRF-CEM cultured cells were treated by resveratrol at doses of 15, 50, and 100 μM based on previous studies. Furthermore, RT Polymerase chain reaction (PCR) was conducted to assess the BAX and BCL-2 gene expression. Moreover, the amount of apoptosis induction was analyzed by annexin V staining method.
Results: Based on the results, time and concentration were found to play a critical role in resveratrol-induced apoptosis. Additionally, BAX upregulation and BCL-2 downregulation excreted by resveratrol in CCRF-CEM cells resulted in predisposing these cells to apoptosis.
Conclusions: In general, it was revealed that resveratrol could have a chemo-preventive activity by modifying the expression of BAX and BCL-2 genes. Finally, resveratrol was found to be a supplement drug in anti-leukemic therapy.
Keywords :
Leukemia , Resveratrol , BCL-2 associated X , BCL-2 Gene , Apoptosis
