Title of article :
A novel method for purification of the synthesized Br-HIDA compound and its identification by FT-IR and HPLC techniques in Pars Isotope Company
Author/Authors :
Nabati, Mehdi Synthesis and Molecular Simulation Laboratory - Chemistry Department - Pars Isotope Company, Tehran, Iran , Mohammadnejad-Mehrabani, Hossein Synthesis and Molecular Simulation Laboratory - Chemistry Department - Pars Isotope Company, Tehran, Iran
Abstract :
In the present article, N-[2,4,6-trimethyl-3-bromoacetanilid]iminodiacetic acid (bromo-HIDA, or Br-HIDA) compound was synthesized in three steps. The Technetium-99m-labeled mebrofenin, transported in blood by binding to albumin in the same manner as the indocyanine green (ICG) clearance test, can be used for hepatobiliary scintigraphy in the assessment of liver function. The important goal of this research work was to purify the Br-HIDA molecule by our novel method (wash with acidic solution and then solvent-antisolvent technique). Then, its purification was identified by HPLC and FT-IR analyses and compared with standard obtained bromo-HIDA. The comparison between the HPLC analyses of our purified mebrofenin and obtained compound shows high accuracy of our method.
Keywords :
Br-HIDA , FT-IR spectroscopy , HPLC technique , Identification , Purification , Radiopharmaceutical
Journal title :
Astroparticle Physics
