Author/Authors :
Lateef, Agbaje Department of Pure and Applied Biology - Ladoke Akintola University of Technology - Ogbomoso, Nigeria , Azeez, Musibau A Department of Pure and Applied Biology - Ladoke Akintola University of Technology - Ogbomoso, Nigeria , Asafa, Tesleem B Department of Mechanical Engineering - Ladoke Akintola University of Technology - Ogbomoso, Nigeria , Yekeen, Taofeek A Department of Pure and Applied Biology - Ladoke Akintola University of Technology - Ogbomoso, Nigeria , Akinboro, Akeem Department of Pure and Applied Biology - Ladoke Akintola University of Technology - Ogbomoso, Nigeria , Oladipo, Iyabo C Department of Science Laboratory Technology - Ladoke Akintola University of Technology - Ogbomoso, Nigeria , Azeez, Luqmon Department of Chemical Sciences - Osun State University - Osogbo, Nigeria , Ojo, Sunday A Department of Pure and Applied Biology - Ladoke Akintola University of Technology - Ogbomoso, Nigeria , Gueguim-Kana, Evariste B Department of Microbiology - University of KwaZulu-Natal, South Africa , Beukes, Lorika S School of Life Sciences - University of KwaZulu-Natal, South Africa
Abstract :
The present investigation reports utility of cocoa
pod husk extract (CPHE), an agro-waste in the biosynthesis
of silver nanoparticles (AgNPs) under ambient condition.
The synthesized CPHE-AgNPs were characterized by UV–
visible spectroscopy, Fourier-transform infrared spectroscopy,
Energy dispersive X-ray (EDX) spectroscopy and
transmission electron microscopy. The feasibility of the
CPHE-AgNPs as antimicrobial agent against some multidrug-
resistant clinical isolates, paint additive, and their
antioxidant and larvicidal activities were evaluated. CPHEAgNPs
were predominantly spherical (size range of
4–32 nm) with face-centered cubic phase and crystalline
conformation pattern revealed by selected area electron
diffraction, while EDX analysis showed the presence of
silver as a prominent metal. The synthesized nanoparticles
effectively inhibited multidrug-resistant isolates of Klebsiella
pneumonia and Escherichia coli at a concentration of
40 lg/ml, and enhanced the activities of cefuroxime and
ampicillin in synergistic manner at 42.9–100 % concentration,
while it completely inhibited the growth of E. coli,
K. pneumoniae, Streptococcus pyogenes, Staphylococcus
aureus, Pseudomonas aeruginosa, Aspergillus flavus,
Aspergillus fumigatus and Aspergillus niger as additive in
emulsion paint. The antioxidant activities of the CPHEAgNPs
were found to be excellent, while highly potent
larvicidal activities against the larvae of Anopheles mosquito
at 10–100 lg/ml concentration were observed. Our
study demonstrated for the first time the utility of CPHE in
the biosynthesis of CPHE-AgNPs with potential applications
as antimicrobial and larvicidal agents, and paint
additives for coating material surfaces to protect them
against microbial growth while improving their shelf life.
Keywords :
CPHE-AgNPs , Antimicrobial activity , Multidrug resistance , Paint additive , Antioxidant , Larvicidal
