Spectrophotometric determination of Dalacin phosphate from formulations using reduce atomic absorption of calcium ion by phosphate

A simple, sensitive and accurate method for spectrophotometric determination of Dalacinphosphate has been established. The method is based on the reduction of atomic absorption calcium ion by phosphate. Because, there is a phosphate mole in every Dalacin mole structure and with measurement of phosphate, Dalacin is beyond measurement too. Therefore, the reaction between acidic hydrolysis products of antibiotics with the mixture of calcium ion evaluated for the spectrophotometric determination of mentioned drug in formulations. Experimental parameters related to the performance of atiomic absorption spectrometry (AAS), such as concentration of calcium ion, reaction time and temperature were investigated and optimized. Under the optimized conditions, the limit of detection (3σB) and relative standard deviation (RSD, n=4) were 1.75 μg mL-1 and 1.4 %, respectively. The results obtained agreed with those obtained by the USP method.