DNA-binding activity of secondary metabolites from SC-CO2 extracts of cat’s whiskers Orthosiphon aristatus (Blume) Miq.

Orthosiphon aristatus is a member of Family Lamiaceae. It is traditionally used for treatment of renal inflammation, kidney disorders and various diseases. To substantiate the pharmacological claims, the plant bioactive components were screened. This study aims to investigate SC-CO2 extracts from leaves of O. aristatus as a potential source of DNA-binding secondary metabolites. One-dimensional thin layer chromatography (TLC) was used to separate the metabolites from the extracts and the binding properties towards DNA were analyzed by two-dimensional TLC. Results showed that all leave extracts from O. aristatus possessed secondary metabolites with binding affinity towards salmon sperm DNA as revealed by their Rf2/Rf1 ratios below 1, thus it can be a source of DNA-binding drugs