Author/Authors :
Pahila, Ida G Department of Chemistry - College of Arts and Sciences - University of the Philippines Visayas - Miagao - Iloilo, Philippines , Calawigan, Roland J. C Department of Chemistry - College of Arts and Sciences - University of the Philippines Visayas - Miagao - Iloilo, Philippines , Toledo, Nieves A Institute of Aquaculture - College of Fisheries and Ocean Sciences - University of the Philippines Visayas - Miagao, Iloilo, Philippines , Garibay, Soledad S Institute of Aquaculture - College of Fisheries and Ocean Sciences - University of the Philippines Visayas - Miagao, Iloilo, Philippines , Jimenez, Caridad N Institute of Fisheries Policy and DevelopmentStudies - College of Fisheries and Ocean Sciences - University of the Philippines Visayas - Miagao - Iloilo, Philippines , Ami, Leticia J Institute of Fish Processing Technology - College of Fisheries and Ocean Sciences - University of the Philippines Visayas - Miagao - Iloilo, Philippines , Mueda, Rose T Institute of Fish Processing Technology - College of Fisheries and Ocean Sciences - University of the Philippines Visayas - Miagao - Iloilo, Philippines , Paulines, Mellie J. S Department of Chemistry - College of Arts and Sciences - University of the Philippines Visayas - Miagao - Iloilo, Philippines , Retiracion, Pilar M. A Division of Professional Education - College of Arts and Sciences - University of the Philippines Visayas - Miagao - Iloilo, Philippines
Abstract :
This study was conducted to compare lipid extraction from wet paste-like Tetraselmis sp.
using isopropanol:hexane, absolute ethanol and chloroform:methanol at different ultrasonic extraction
times. The best solvent system and ultrasonic extraction time is chloroform:methanol (1:1) at 50
minutes, respectively. Soaking effect (before and after extraction) gave a higher crude lipid (0.65 % -
28 %) recovery than those that were not. Ultrasonic extraction of Tetraselmis sp. gave higher crude
lipid yield (15.3 %) than the Folch method (11.78 %). Qualitative analysis of fatty acid profile using
different solvents showed no significant difference. Fatty acid present in Tetraselmis sp. is
predominantly C16 (Palmitic acid) with significant amount of C18:0 (Stearic acid), C18:1 n-9 (Oleic acid),
C18:2 n-6 (Linoleic acid) and C18:3 n-3 (α-Linolenic acid) and C20:5 n-3 (Eicosapentanoic acid). Fatty acid profile
is not dependent on extraction solvent but crude lipid yield varies with the solvent system.
Keywords :
isopropanol:hexane , absolute ethanol , chloroform methanol , soaking effect
