Author/Authors :
Ibrahim, Majid A University of Basra - Iraq , Jerry, Awatif N University of Basra - Iraq , Khalil, Amani I University of Basra - Iraq
Abstract :
The callus of Lizita cultivar gave the highest significant value in its content of proteins, proline
and malondialdehyde (MDA) compared to the Arnova and Safari cultivars as it reached 16.23 mg g
-1 dw, 3.05 µg g -1 dw and 2.04 nmol g -1 fw, respectively. Also the callus gave the highest significant value in its content of ascorbic acid, total dissolved carbohydrates, proline and MDA when cultured on MS medium
supplemented with 80 mmol L -1 of NaCl as it reached 12.91 mg 100g-1 fw, 32.45 mg g -1 dw, 3.41 µg g
-1 dw and 2.46 nmol g -1 fw, respectively. But it gave the lowest significant value in its content of total
proteins when cultured on MS medium supplemented with 80 mmol L -1 of NaCl, reached 12.57 mg g -1
dw. The interaction between Lizita cultivar and 80 mmol L -1 gave the highest significant value in total
dissolved carbohydrates; proline and MDA accumulation reached 33.18 mg g -1 dw, 4.15 µg g -1 dw and
3.17 nmol g -1 fw, respectively. While the Lizita cultivar and 0.0 mmol L -1 NaCl treatment gave the highest
significant value in total proteins content reached 18.21 mg g -1 dw. The callus of Lizita cultivar gave the
highest significant value in its content of proteins, proline and MDA compared to the Arnova cultivar
when cultured on MS medium supplemented with 100 mmol L -1 of NaCl, as it reached 12.6 mg g -1 dw, 5.04 µg g -1 dw and 4.61 nmol g -1 fw, respectively.
Keywords :
ascorbic acid , benzyl adenine , in vitro , lipid peroxidation , MS salts