Author/Authors :
Salehinodeh, AR Dept. of Medical Immunology - School of Public Health - Tehran University of Medical Sciences ,Tehran , Ekhtiyari, P Dept. of Medical Immunology - School of Public Health - Tehran University of Medical Sciences ,Tehran , Mirshafiey, A Dept. of Medical Immunology - School of Public Health - Tehran University of Medical Sciences ,Tehran , Rezai, S Dept. of Medical Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , Javanmard-Khamene, H Dept. of Medical Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran Dept. of Medical Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran
Abstract :
Background: Trichophyton verrucosum is a zoophilic fungus with a worldwide distribution. Our aim was to investigate
the proliferative effect of antigenic compounds of T. verrucosum on dermis fibro blasts and endothelial cells.
Methods: T. verrucosum was cultured in SCC medium and was then transferred to a broth medium. Surface antigens
of this fungus were separated using the freeze and thaw method. The sample was centrifuged and the supernatant
was taken. The supernatant was homogenized and purified. The prepared antigenic extract was added
to fibro blast cell lines according to a regular timetable. Cytotoxicity and cell proliferation were evaluated using
zymography and densitometry in order to assay MMPs activity.
Results: Statistical analyses showed that this antigenic extract is able to enhance the MMPs activity
Conclusion: Trichophyton verrucosum increases the proliferation of dermis germinal layer and MMP-2 activity,
which has a direct relation with wound healing process.
