Title of article :
Candida parapsilosis as a Potent Biocontrol Agent against Growth and Aflatoxin Production by Aspergillus Species
Author/Authors :
Niknejad, F Dept. of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Zaini, F Dept. of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Kordbacheh, P Dept. of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Safara, M Dept. of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Faramarzi, MA Dept. of Pharmaceutical Biotechnology - School of Pharmacy and Biotechnology Research Center - Tehran University of Medical Sciences , Amini, M Dept. of Medicinal Chemistry - School of Pharmacy - Tehran University of Medical Sciences, Tehran , Mahmoudi, M Dept. of Epidemiology and Biostatistics - School of Public Health - Tehran University of Medical Sciences
Abstract :
Background: Aflatoxin contamination of food and feed stuff is a serious health problem and significant economic
concerns. In the present study, the inhibitory effect of Candida parapsilosis IP1698 on mycelial growth and aflatoxin
production in aflatoxigenic strains of Aspergillus species was investigated.
Methods: Mycelial growth inhibitions of nine strains of aflatoxigenic and non-aflatoxigenic Aspergillus species in the
presence of C. parapsilosis investigated by pour plate technique at different pH, temperature and time of incubation.
Reduction of aflatoxin was evaluated in co-cultured fungi in yeast extract sucrose broth after seven days of incubation
using HPLC method. The data were analyzed by SPSS 11.5.
Results: The presence of the C. parapsilosis at different pH did not affect significantly the growth rate of Aspergillus
isolates. On the other hand, temperature and time of incubation showed to be significantly effective when compared
to controls without C. parapsilosis (P≤0.05). In aflatoxigenic strains, minimum percentage of reductions in total
aflatoxin and B1, B2, G1, G2 fractions were 92.98, 92.54, 77.48, 54.54 and 72.22 and maximum percentage of
reductions were 99.59, not detectable, 94.42, and not detectable in both G1 and G2, respectively.
Conclusion: C. parapsilosis might employ as a good biocontrol agent against growth and aflatoxin production by
aflatoxigenic Aspergillus species
Keywords :
Biocontrol , Candida parapsilosis , Detoxification , Aflatoxin B1 , HPLC
Journal title :
Astroparticle Physics
