Title of article :
Cytotoxic Activity of Familact: A Probiotic Supplement
Author/Authors :
Yahyavi, Zahra Department of Microbiology - Karaj Branch - Islamic Azad University, Alborz , Fazeli, Mohammad Reza Department of Drug & Food Control - Tehran University of Medical Sciences, Tehran , Mirfeizi, Mani Department of Midwifery - College of Nursing and Midwifery - Karaj Branch - Islamic Azad University, Karaj , Aliebrahimi, Shima Department of Toxicology and Pharmacology - Tehran University of Medical Sciences, Tehran , Ostad, Nasser Department of Toxicology and Pharmacology - Tehran University of Medical Sciences, Tehran
Abstract :
Background: Lactobacillus and Bifidobacterium species are among the probiotics discussed due to
their anti-cancer effects in the treatment of colorectal and breast cancers in recent studies. The aim
of this study was to investigate the anticancer effect of Familact, a commercial probiotic capsule
containing seven bacterial strains (L. casei, L. acidophilus, L. rhamnosus, L. bulgaricus, B. breve, B.
longum and Streptococcus thermophilus).
Methods: Various cancer cell lines including Caco-2, HT-29, T47D and normal cell line L929 were
treated with different concentrations of Familact. Using MTT assay, the cytotoxicity effect was
investigated for each cell line and then flow cytometry analysis of apoptosis was evaluated.
Results: Familact demonstrated inhibitory effects on the proliferation of all tested cancer cell lines in
a dose-dependent manner. Although Familact augmented apoptotic cell death in HT-29 human
cancer cells, it was less effective in the case of Caco-2 and T47D cells. Moreover, exposure to
Familact showed moderate cytotoxicity towards L929 mouse fibroblast cells.
Conclusion: Familact could be considered as a complementary therapy in the treatment of cancers.
Keywords :
Apoptosis , Breast Cancer , Colorectal Cancer , Familact , Prebiotic , Probiotics
Journal title :
Astroparticle Physics
