Author/Authors :
Miladpoor, B Department of Clinical Biochemistry - Fasa University of Medical Sciences, Fasa, Iran , Behravan, J Biotechnology and Pharmaceutical Research Center , Nejatshokouhi, A Department of Clinical Biochemistry , Banihashem, A Department of Oncology , Smaili, H Department of Statistics - Mashhad University of Medical Sciences, Mashhad, Iran , Khedri, A Islamic Azad University - Masjed Soleyman , Meshkibaf, MH Islamic Azad University - Masjed Soleyman , Meshkibaf, MH Department of Clinical Biochemistry - Fasa University of Medical Sciences, Fasa, Iran , Ataollahi, MR Department of Clinical Biochemistry - Fasa University of Medical Sciences, Fasa, Iran
Abstract :
Background: P-glycoprotein (P-gp), an ATP-dependent efflux pump, is a membrane protein encoded by MDR1
gene. P-gp has an important role in protection of the cell against xenobiotics and toxic compounds. Recently, a
silent C3435T polymorphism in exon 26 of MDR1 has been reported to be associated with a decreased expression
of P-gp in TT genotypes carriers compared with CC genotypes carriers.
Methods: To evaluate the distribution of allelic variants of C3435T MDR1 in a group of healthy population in Iran
and find the association between MDR1 C3435T polymorphism and the incidence of ALL, 126 patients with ALL
and 139 healthy controls were included in our study and their MDR1 polymorphisms were detected by PCRRFLP
assay.
Results: 71.9% of the healthy people had 3435TC genotype, 15.8% had 3435TT genotype and 12.2% had
3435CC genotype. Also, the frequency of T allele was 51.8% and C allele 48.2%. The mutant homozygous TT
and TC genotypes were found to be associated with the incidence of ALL (OR=1.96 for TT genotype and
OR=0.53 for TC genotype).
Conclusion: MDR1 C3435T polymorphism may contribute to the incidence of ALL. TT genotypes carriers are
more at risk of developing ALL than other genotypes carriers.
Keywords :
P-glycoprotein , MDR1 , Acute lymphoblastic leukemia , C3435T polymorphism
