Title of article :
Molecular epidemiology of colistin-resistant Pseudomonas aeruginosa producing NDM-1 from hospitalized patients in Iran
Author/Authors :
Shahin, Mojtaba Department of Microbiology - Faculty of Medicine - Ahvaz Jundishapur University of Medical Sciences - Ahvaz, Iran , Farajzadeh Sheikh, Ahmad Department of Microbiology - Faculty of Medicine - Ahvaz Jundishapur University of Medical Sciences - Ahvaz, Iran , Shokoohizadeh, Leili Department of Microbiology - Faculty of Medicine - Hamadan University of Medical Sciences - Hamadan, Iran , Halaji, Mehrdad Department of Microbiology - School of Medicine - Isfahan University of Medical Sciences - Isfahan, Iran , Shahcheraghi, Fereshteh Department of Bacteriology - Microbiology Research Center - Pasteur Institute of Iran - Tehran, Iran , Ghanbari, Fahimeh Student Research Committee - School of Medicine - Shahid Saddoughi University of Medical Sciences - Yazd, Iran
Abstract :
Resistance to carbapenems is the principal reason for the continuing utilization of colistin
as a last resort choice for treating the infections resulted from multidrug carbapenem-resistant
Pseudomonas aeruginosa (CRPA) isolates. The assessment of antimicrobial resistance pattern, the
prevalence of carbapenem-resistance determinants, and molecular epidemiology of colistin-resistant
isolates among CRPA strains were the aims of the present research.
Materials and Methods: The current cross-sectional research was conducted on 269 CRPA isolates collected
from various clinical samples from 2013 to 2016. After performing identification tests, disk diffusion as well
as MIC methods were used for testing sensitivity to the antibiotics. Modified Hodge Test (MHT) was utilized
to produce carbapenemase. PCR technique identified beta-lactamase classes A, B, and D genes.
Results: In total, from 269 CRPA, five isolates (1.3%) were resistant to colistin. It was found that
blaNDM-1, blaIMP-1, blaVIM-2, and blaOXA-10 genes were present in 40%, 40%, 20%, and 100% of
colistin-resistant isolates, respectively. DLST type 25-11 is a significant cluster of colistin-resistant P.
aeruginosa isolates.
Conclusion: The appearance of colistin-resistant isolates in CRPA carrying blaNDM-1 with multiple
carbapenem-resistant genes shows the great problem in the treatment of P. aeruginosa infections
Keywords :
Pseudomonas aeruginosa , bla NDM-1 , Colistin , Double-locus sequence typing , Drug resistance
Journal title :
Astroparticle Physics
