Title of article :
The role of locus coeruleus nucleus TRPV1 receptors in the development and expression of morphine dependence
Author/Authors :
Fatemi, Iman Research Center of Tropical and Infectious Diseases - Kerman University of Medical Sciences , Hadadianpour, Zahra Physiology-pharmacology Research Center - Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences , Fatehi, Farangis Physiology-pharmacology Research Center - Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences , Shamsizadeh, Ali Physiology-pharmacology Research Center - Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences - Department of Physiology and Pharmacology - Rafsanjan University of Medical Sciences , Hasanshahi, Jalal Physiology-pharmacology Research Center - Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences - Department of Physiology and Pharmacology - Rafsanjan University of Medical Sciences , Abbasifard, Mitra Department of Internal Medicine - Ali-Ebn Abitaleb Hospital - Rafsanjan University of Medical Sciences , Kaeidi, Ayat Physiology-pharmacology Research Center - Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences - Department of Physiology and Pharmacology - Rafsanjan University of Medical Sciences
Abstract :
Objective(s): This study investigated the role of locus coeruleus (LC) nucleus TRPV1 receptors (TRPV1r)
in the expression and development of morphine physical dependence by intra-LC administration of
AMG9810 (selective TRPV1r antagonist) in male Wistar rats.
Materials and Methods: For assessing the development of morphine dependence, AMG9810 (0.03 and 0.3
mM in 10% DMSO, 0.2 μl; intra-LC microinjection) was administered before each morphine administration
for seven continues days (once daily; 6, 16, 26, 36, 46, 56, and 66 mg/kg; sc). Furthermore, for evaluating
the expression of morphine dependence, a single dose of AMG9810 (0.03 and 0.3 mM in 10% DMSO, 0.2 μl;
intra-LC microinjection) was administered to morphine-dependent rats on day 8 of the experiment.
Results: Obtained data demonstrated that co-administration of TRPV1r antagonist with morphine
reduced the development of morphine withdrawal syndrome somatic signs induced by naloxone.
Moreover, single intra-LC administration of TRPV1r antagonist on the final day of the examination
period significantly decreased the expression of some signs of morphine withdrawal in rats.
Conclusion: The results showed that LC TRPV1r might be participating in the expression and
development of morphine dependence.
Keywords :
Dependence , Locus coeruleus , Morphine , Rat , TRPV1
Journal title :
Astroparticle Physics
