The Effect of Testosterone and Estradiol on Renal Function Markers in Two Protocols of Cisplatin Induced Nephrotoxicity Models in Surgically Orchiectomized and Ovariectomized Rats

Background: The major side effect of cisplatin (CP) therapy in patients with cancer is nephrotoxicity, which limits the treatment. In two protocols of CP treatments, we tested 3 weeks of hormone therapy against CP induced nephrotoxicity in bilateral orchiectomized (OR) and ovariectomized (OV) rats. Methods: A total of 101 o‎r and OV rats were subjected to receive 3 weeks of testosterone (Ts, 10 mg/kg/week) and estradiol (Es, 250 g/kg/week), respectively, followed by two protocols of CP therapies; continuous (divided) doses (3 mg/kg/day for period of 5 days) as protocol 1 and single dose (7.5 mg/kg) as protocol 2. The measurements were performed by the end of the 4th week. Results: CP increased the serum levels of blood urea nitrogen (BUN) and creatinine (Cr) in both o‎r and OV rats, which were related to the protocols of CP treatments. Es (not Ts) in protocol 2 attenuated the serum levels of BUN and Cr. Ts significantly increased body weight loss in protocol 1 compared with control group (P < 0.05). Es did not attenuate kidney tissue damage score (KTDS) in protocol 1 treated animal, but KTDS was decreased by Es in protocol 2 treated Rats. Hormone replacement therapy did not alter Cr clearance compared with control group. Conclusions: It seems that hormone therapy could not protect the kidney against CP induced nephrotoxicity.