Title of article :
miR-4284 and miR-4484 as Putative Biomarkers for Diffuse Large B-Cell Lymphoma
Author/Authors :
Tamaddon, Gholamhossein Department of Hematology - School of Medical Sciences - Tarbiat Modares University - Tehran , Geramizadeh, Bita Department of Pathology - School of Medicine - Shiraz University of Medical Sciences - Shiraz , Karimi, Mohammad Hossein Transplant Research Center - Nemazee Teaching Hospital - Shiraz University of Medical Sciences - Shiraz , Mowla, Javad Department of Genetics - Faculty of Basic Sciences - Tarbiat Modares University - Tehran , Abroun, Said Department of Hematology - School of Medical Sciences - Tarbiat Modares University - Tehran
Abstract :
Diffuse large B-cell lymphoma is the most common type of non-
Hodgkin lymphoma. MicroRNAs (miRNAs) are endogenous
small RNA, which can regulate gene expression at the posttranscriptional
level. MiRNA profiling has shown a great
potential as novel diagnostic and prognostic biomarkers. The
present study was performed at the Nemazee Teaching Hospital
(Shiraz, Iran) from 2011 to 2013.The aim of this study was to
assess the deregulation of miRNAs profiles in DLBL against
hyperplasic reactive lymph node as a normal. This could serve
as a biomarker for DLBL. The miRCURY LNATM microarray
was used on the total RNA, which was extracted from formalinfixed
paraffin-embedded tissue of 24 de novo diffuse large
B-cell lymphoma patients and 14 normal lymph nodes. The
greatest changes were detected in miR-4284 and miR-4484
level in patient’s lymphoma samples. These miRNAs can act as
a diagnostic biomarker for DLBL.
Keywords :
Lymphoma , Large B-Cell , Diffuse , MicroRNAs , Microarray Analysis
Journal title :
Astroparticle Physics
