Benidipine reduces ischemia/reperfusion injury following testicular torsion/detorsion in rats

Objective- This study aimed to determine the protective effects of benidipine on testicular injury after testis torsion/detorsion in rats. Design-Experimental study Animals-Fifteen male Sprague-Dawly rats weighing 200-220g Procedure- Rats were randomly divided into 3 groups: sham (healthy group with sham operation, n=5), IRC group (control group in which I/R injury was performed by torsion the right testis 720 º clockwise for 2 hours and detorsion for 2 hours, n=5), and IRB group (IR + benidipine, I/R process was performed following orally benidipine administration + 4 mg/kg benidipine, n=5). Levels of glutathione peroxidase (GPx) and malondialdehyde (MDA) were determined in testicular tissues of rats. Testicular tissues were also examined histopathologically. Results- The levels of MDA (P<0.0001) was significantly increased augmented in the testis of IRC group rat. Testicular GPx levels were significantly reduced (p<0.0001) after I/R. Administration of benidipine before torsion prevented the increase in lipid peroxidation and alleviated GPx levels. Benidipine also impeded ischemia/reperfusion cellular damage and histological alternations in testicular tissue. Conclusion and clinical relevance- These results show that therapy with benidipine before torsion may induce protective effects against ischemia/reperfusion injury.