Title of article
Prevalence of Aminoglycoside Resistance Genes in Enterococcus Strains in Kermanshah, Iran
Author/Authors
Amini, Fatemeh Department of Medical Microbiology, Research Assistant in Kermanshah University of Medical Sciences, Kermanshah, , Krimpour, Hasan Ali Department of Anesthesiology - Medical Faculty, Kermanshah University of Medical Sciences , Ghaderi, Mahdi Department of Microbiology, Boroujerd Branch, Islamic Azad University, Boroujerd , Vaziri, Siavash Department of Microbiology - School of Medicine, Kermanshah University of Medical Sciences , Azizi, Mohsen Department of Microbiology - School of Medicine, Kermanshah University of Medical Sciences , Ferdowsi, Shirin Department of Hematology - Blood Transfusion Research Center - High Institute for Research and Education in Transfusion Medicine , Amini, Sabrieh Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj
Pages
7
From page
487
To page
493
Abstract
Background: This study aimed to investigate the occurrence of
aminoglycoside resistance and the prevalence of the resistancemodifying
enzyme genes, ant(3”)-III, ant(6’)-Ia, aac(6′)-Ieaph(
2”)-Ia, and aph(2’)-Id, in Enterococcus strains isolated in
Kermanshah Province, west of Iran.
Methods: In this cross-sectional study, 108 enterococcal isolates
from urine, wound, blood, and cerebrospinal fluid samples were
collected. The Enterococcus species were recognized by standard
phenotypic/biochemical tests. The antimicrobial resistance forms
were detected using a disc diffusion method. Polymerase chain
reaction was designed to identify aminoglycoside resistance
genes, including ant(3”)-III, ant(6’)-Ia, aac(6′)-Ie-aph(2”)-Ia,
and aph(2’)-Id.
Results: Totally, 108 strains with a final diagnosis of Enterococcus
were extracted from 84 (77.8%) urine, 14 (13%) wound, 6 (5.6%)
blood, and 4 (3.7%) cerebrospinal fluid samples. Among the 108
Enterococcus specimens, 94 (87%) cases were Enterococcus
faecalis and 14 (13%) were Enterococcus faecium. The highest
frequency of resistance was observed for erythromycin (88.9%),
while the lowest was found for streptomycin (44.4%). The
frequency of high-level gentamicin resistance was 42.2%.
Among the identified specimens, 42.6% contained the aac(6′)-
Ie-aph(2”)-I gene, 20.4% contained the ant(6’)-Ia gene, and
15.7% contained the ant(3”)-III gene. A significant correlation
was found between phenotypic gentamicin resistance and the
presence of the aminoglycoside resistance genes (P<0.05).
Conclusion: This study showed the high resistance of
Enterococcus strains isolated from hospital samples. Compared
with the previous studies, the strains isolated in our study showed
a higher percentage of resistance to aminoglycosides.
Keywords
Aminoglycosides , Enterococcus , Aminoglycoside resistance genes , Prevalence
Journal title
Astroparticle Physics
Serial Year
2018
Record number
2445734
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