Title of article
Effects of Alpha- Tocopherol on the Velocity of Low Density Lipoprotein Oxidation by Cupric Ions
Author/Authors
Ghaffari, Mohammad Ali Cellular and Molecular Research Center and Department of Biochemistry - School of Medical - Ahwaz Jundishapour University of Medical Sciences, Ahwaz , Ghiasvand, Taibeh Cellular and Molecular Research Center and Department of Biochemistry - School of Medical - Ahwaz Jundishapour University of Medical Sciences, Ahwaz
Pages
6
From page
289
To page
294
Abstract
We studied the effect of different concentrations of alpha-tocopherol on in vitro cupric ions
induced oxidation of low density lipoproteins (LDL). Human native LDL (50 μg protein/ml) oxidation was
induced by 10 μmol/L of CuSO4. Conjugated dienes were measured spectrophotometrically for up to 440
minutes. The length of the lag phase (Tlag), maximum velocity of the reaction (Vmax) and the maximum
amount of generated dienes were obtained from kinetic data. Alpha-tocopherol increased Tlag and decreased
Vmax with a dependence upon concentration (0-100 μmol/L). There was no difference between the Dmax
obtained with cupric ions alone or in the presence of the various concentrations of alpha-tocopherol. The
results suggest that alpha-tocopherol may decrease free radicals presence in LDL and thus decrease velocity
of LDL oxidation by cupric ions. This mechanism may be a reason for alpha-tocopherol effect in ameliorating
atherosclerosis.
Keywords
Lipoprotein , LDL , cupric ions , alpha- Tocopherol , oxidation- reduction
Journal title
Astroparticle Physics
Serial Year
2010
Record number
2445778
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