Other language title :
ﺑﻬﺒﻮد ﻋﻤﻠﻜﺮد ﻗﻠﺐ ﭘﺲ از ﭘﻴﻮﻧﺪ ﺳﻠﻮلﻫﺎي ﻣﺰاﻧﺸﻴﻤﻲ ﺧﻮدي ﻣﺸﺘﻖ از ﺑﺎﻓﺖ ﭼﺮﺑﻲ ﭘﻴﺶ-ﺗﻤﺎﻳﺰ ﻳﺎﻓﺘﻪ ﻗﻠﺒﻲ ﺗﺤﺖ ﺗﺎﺛﻴﺮ ﺑﺎرﮔﺬاري ﻣﻜﺎﻧﻴﻜﻲ در اﻧﻔﺎرﻛﺘﻮس ﺣﺎد ﻣﻴﻮﻛﺎرد در ﻣﺪل ﺣﻴﻮاﻧﻲ ﺧﺮﮔﻮش
Title of article :
Transplantation of Cardiogenic Pre-Differentiated Autologous Adipose-Derived Mesenchymal Stem Cells Induced by Mechanical Loading Improves Cardiac Function Following Acute Myocardial Infarction in Rabbit Model
Author/Authors :
Mahmoudi, Elena Department of Surgery and Radiology - Faculty of Veterinary Medicine - University of Tehran , Dehghan, Mohammad Mehdi Department of Surgery and Radiology - Faculty of Veterinary Medicine - University of Tehran , Shokrgozar, Mohammad Ali National Cell Bank of Iran - Pasteur Institute of Iran , Emami, Baharak National Cell Bank of Iran - Pasteur Institute of Iran , Tafazoli-Shadpour, Mohammad Department of Biomedical Engineering - Amirkabir University of Technology , Amin, Suzan Department of Biomedical Engineering - Amirkabir University of Technology
Abstract :
Objective- Investigate myocardial performance after autologous adipose-derived (ASCs) mesenchymal
stem cell differentiated under equiaxial cyclic strain, transplantation in rabbits with acute myocardial
infarction (AMI).
Design- Prospective, randomized experimental study
Animals- 20 New Zealand White rabbits (2-3 kg)
Procedure- ASCs were studied in four distinct groups of mechanical (ADM), chemical (ADC),
undifferentiated (AD) and control (C) groups. According to this categorization, the cells were exposed to
cyclic mechanical loading or 5-azacytidine as the chemical factor. 10 6 ASC cells were transplanted
intramyocardially in rabbits with AMI (Acute Myocardial Infarction). Echocardiographic study was used
to evaluate effects of cells on cardiac function.
Results- Left ventricle ejection fraction (LVEF) was significantly increased in the ADM (mechanicallydifferentiated
adipose-derived mesenchymal stem cell) group at 2 months follow-up. Fractional
shortening (FS) also showed a similar pattern as LVEF and increased in ADM group in compare to
control and AD (undifferentiated adipose-derived mesenchymal stem cell) group.
Conclusion and clinical prevalence- The results indicate that intramyocardial transplantation of
mechanically-differentiated ASCs improves cardiac function of ischemic myocardium. Transplantation of
mechanically-differentiated ASCs for myocardial regeneration may become the future therapy for acute
myocardial infarction
Farsi abstract :
ﻫﺪف- ﺑﺮرﺳﻲ ﻛﺎراﻳﻲ ﻣﻴﻮﻛﺎرد ﻗﻠﺐ ﺑﻪ دﻧﺒﺎل ﭘﻴﻮﻧﺪ ﺳﻠﻮل ﻣﺰاﻧﺸﻴﻤﻲ ﻣﺸﺘﻖ از ﭼﺮﺑﻲ ﻛﻪ ﺗﺤﺖ ﺑﺎر ﻣﻜﺎﻧﻴﻜﻲ ﻗﺮار ﮔﺮﻓﺘﻨﺪ، در اﻧﻔﺎرﻛﺘﻮس ﺣﺎد ﻗﻠﺒﻲ ﺧﺮﮔﻮش
ﻃﺮح ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﻲ آﻳﻨﺪه ﻧﮕﺮ
ﺣﻴﻮاﻧﺎت- 20 ﺳﺮ ﺧﺮﮔﻮش ﺳﻔﻴﺪ ﻧﻴﻮزﻟﻨﺪي )وزن 2 ﺗﺎ 3 ﻛﻴﻠﻮﮔﺮم(
روش ﻛﺎر- ﺳﻠﻮلﻫﺎي ﻣﺰاﻧﺸﻴﻤﻲ ﻣﺸﺘﻖ از ﭼﺮﺑﻲ در ﭼﻬﺎر ﮔﺮوه ﻣﺠﺰا ﺷﺎﻣﻞ ﮔﺮوه ﻣﻜﺎﻧﻴﻜﻲ، ﺷﻴﻤﻴﺎﻳﻲ، ﺗﻤﺎﻳﺰ ﻧﻴﺎﻓﺘﻪ و ﺷﺎﻫﺪ ﻣﻄﺎﻟﻌﻪ ﺷﺪﻧﺪ. ﻃﺒﻖ اﻳﻦ دﺳﺘﻪ ﺑﻨﺪي، ﺳﻠﻮلﻫﺎ ﺗﺤﺖ ﻟﻮد ﺑﺎر ﻣﻜﺎﻧﻴﻜﻲ و ﻳﺎ 5-آزاﺳﺎﻳﺘﻴﺪﻳﻦ )ﺑﻪ ﻋﻨﻮان ﻓﺎﻛﺘﻮر ﺷﻴﻤﻴﺎﻳﻲ( ﻗﺮار ﮔﺮﻓﺘﻨﺪ. 106 ﺳﻠﻮل ﻣﺰاﻧﺸﻴﻤﻲ ﻣﺸﺘﻖ از ﭼﺮﺑﻲ داﺧﻞ ﻣﻴﻮﻛﺎرد ﻗﻠﺐ ﺧﺮﮔﻮش ﺑﻪ دﻧﺒﺎل اﻧﻔﺎرﻛﺘﻮس ﺣﺎد ﻣﻴﻮﻛﺎرد ﺗﺰرﻳﻖ ﺷﺪ. ﺑﺮرﺳﻲ اﻛﻮﻛﺎردﻳﻮﮔﺮاﻓﻲ ﺟﻬﺖ ارزﻳﺎﺑﻲ ﻋﻤﻠﻜﺮد ﻗﻠﺒﻲ ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﮔﺮﻓﺖ.
ﻧﺘﺎﻳﺞ -ﻛﺴﺮ ﺟﻬﺸﻲ )Ejection fraction( ﺑﻄﻦ ﭼﭗ ﺑﻌﺪ از ﮔﺬﺷﺖ 2 ﻣﺎه در ﮔﺮوه ﺳﻠﻮلﻫﺎي ﻣﺰاﻧﺸﻴﻤﻲ ﻣﺸﺘﻖ از ﭼﺮﺑﻲ ﺗﻤﺎﻳﺰ ﻳﺎﻓﺘﻪ ﺑﻪ روش ﻣﻜﺎﻧﻴﻜﻲ ﺑﻪ ﻃﻮر ﻣﻌﻨﻲداري اﻓﺰاﻳﺶ ﻳﺎﻓﺖ. ﻛﺴﺮ ﻛﺎﻫﺸﻲ )Fractional shortening( ﻧﻴﺰ ﺑﺎ اﻟﮕﻮﻳﻲ ﻣﺸﺎﺑﻪ ﻛﺴﺮ ﺟﻬﺸﻲ اﻓﺰاﻳﺶ ﻣﻌﻨﻲ-داري ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﻛﻨﺘﺮل و ﺗﻤﺎﻳﺰ ﻧﻴﺎﻓﺘﻪ، ﻧﺸﺎن داد.
ﻧﺘﻴﺠﻪﮔﻴﺮي و ﻛﺎرﺑﺮد ﺑﺎﻟﻴﻨﻲ ﻧﺘﺎﻳﺞ اﻳﻦ ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎن ﻣﻲدﻫﺪ ﻛﻪ ﺳﻠﻮل ﻣﺰاﻧﺸﻴﻤﻲ ﻣﺸﺘﻖ از ﭼﺮﺑﻲ ﺗﻤﺎﻳﺰ ﻳﺎﻓﺘﻪ ﺑﻪ روش ﻣﻜﺎﻧﻴﻜﻲ ﺑﻪ دﻧﺒﺎل ﺗﺰرﻳﻖ داﺧﻞ ﻣﻴﻮﻛﺎرد در اﻧﻔﺎرﻛﺘﻮس ﺣﺎد ﻗﻠﺒﻲ ﺑﺎﻋﺚ ﺑﻬﺒﻮدﻛﺎرﻛﺮد ﻗﻠﺐ ﻣﻲﺷﻮد. ﭘﻴﻮﻧﺪ ﺳﻠﻮلﻫﺎي ﻣﺰاﻧﺸﻴﻤﻲ ﻣﺸﺘﻖ از ﭼﺮﺑﻲ ﺗﻤﺎﻳﺰ ﻳﺎﻓﺘﻪ ﺑﻪ روش ﻣﻜﺎﻧﻴﻜﻲ ﻣﻲ ﺗﻮاﻧﺪ در آﻳﻨﺪه ﺑﺮاي درﻣﺎن اﻧﻔﺎرﻛﺘﻮس ﻣﻴﻮﻛﺎرد ﺑﻪ ﻛﺎر رود.
Keywords :
Adipose-derived mesenchymal stem cell , Equiaxial strain , Acute myocardial infarction , Echocardiography
Journal title :
Astroparticle Physics
