Author/Authors :
Rasoulian, B Razi Herbal Medicines Research Center and Department of Physiology - Lorestan University (Medical sciences) - Khorramabad, Iran , Jafari, M Trauma Research Center - Baqiyatallah University of Medical Sciences - Tehran, Iran , Wahhab-Aghai, H Trauma Research Center - Baqiyatallah University of Medical Sciences - Tehran, Iran , Noroozzadeh, A Department of Physiology and Biophysics - School of Medicine - Baqiyatallah University of Medical Sciences - Tehran, Iran , Ghani, E Department of Physiology and Biophysics - School of Medicine - Baqiyatallah University of Medical Sciences - Tehran, Iran , Asgari, A Department of Physiology and Biophysics - School of Medicine - Baqiyatallah University of Medical Sciences - Tehran, Iran , Khoshbaten, A Department of Physiology and Biophysics - School of Medicine - Baqiyatallah University of Medical Sciences - Tehran, Iran , Mehrani, H Department of Biochemistry - School of Medicine - Baqiyatallah University of Medical Sciences - Tehran, Iran , Hashemi-Madani, S. M. H. Research Center for Molecular Biology - School of Medicine - Baqiyatallah University of Medical Sciences - Tehran, Iran , Esmaili, M Department of Physiology - School of Medicine - Yazd University of Medical Sciences - Yazd, Iran
Abstract :
Renal ischemia-reperfusion (IR) injury is a common problem in kidney transplantation. There is increasing evidence about the role of the reactive oxygen species (ROS) in these injuries and endogenous antioxidants seem to have an important role in decreasing the renal tissue injury. The aim of this study was to investigate the effects of kidney IR on renal antioxidant enzymes activity such as catalase and superoxide dismutase (SOD) and renal glutathione (GSH) levels. The level of malondialdehyde (MDA), an end product of lipid peroxidation, also was quantified. Male Wister rats (200-280 g) were anesthetized and after right nephrectomy, the left renal artery was clamped for 40 min. After 24 h reperfusion, serum and kidney tissue samples were obtained for metabolites assay. Results showed that renal IR increased serum creatinine and urea level [90.59 ± 12.93 vs. 44.06 ± 4.07 μmol/L (P < 0.01), and 40.22 ± 10.27 vs. 20.8 ± 1.8 mmol/L, (P < 0.05), respectively]. The renal catalase activity was decreased (33.13 ± 2.02 vs. 43.78 ± 2.38 units/mg protein, P < 0.01) but SOD activity was increased (57.59 ± 4.64 vs. 42.84 ± 1.85 units/mg protein, P < 0.05). The GSH level also was decreased (24.67 ± 2.31 vs. 37.44 ± 3.09 nmol/mg protein P < 0.01). MDA level after IR was not significantly different from control group (2.05 ± 0.27 vs. 1.84 ± 0.2 nmol/mg protein, P = 0.55). Our data indicated that despite decrease in renal GSH level and catalase activity following IR, SOD activity was increased.
Keywords :
malondialdehyde , antioxidant , reperfusion , ischemia , Kidney
