Title of article :
Barriers to Liposomal Gene Delivery: from Application Site to the Target
Author/Authors :
Saffari, Mostafa Department of Pharmaceutics and Nanotechnology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Moghimi, Hamid Reza Department of Pharmaceutics and Nanotechnology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Dass, Crispin School of Pharmacy - Faculty of Health Sciences - Curtin University, Perth, Australia
Abstract :
Gene therapy is a therapeutic approach to deliver genetic material into cells to alter their
function in entire organism. One promising form of gene delivery system (DDS) is liposomes.
The success of liposome-mediated gene delivery is a multifactorial issue and well-designed
liposomal systems might lead to optimized gene transfection particularly in vivo. Liposomal
gene delivery systems face different barriers from their site of application to their target,
which is inside the cells. These barriers include presystemic obstacles (epithelial barriers),
systemic barriers in blood circulation and cellular barriers. Epithelial barriers differ depending
on the route of administration. Systemic barriers include enzymatic degradation, binding and
opsonisation. Both of these barriers can act as limiting hurdles that genetic material and their
vector should overcome before reaching the cells. Finally liposomes should overcome cellular
barriers that include cell entrance, endosomal escape and nuclear uptake. These barriers and
their impact on liposomal gene delivery will be discussed in this review
Keywords :
Gene therapy , Drug delivery , Liposomes , Epithelial barriers , Cellular uptake and release , Degradation , Targeting
Journal title :
Astroparticle Physics