Author/Authors :
Boğa, Mehmet Department of Pharmaceutical Technology, Faculty of Pharmacy, Dicle University, 21280 Diyarbakır, Turkey , Ertaş, Abdulselam Department of Pharmacognosy, Faculty of Pharmacy, Dicle University, 21280 Diyarbakır, Turkey , Abdullah Yılmaz, Mustafa cResearch and Application of Science and Technology Center (DUBTAM), Dicle University, 21280 Diyarbakir, Turkey , Kızıl, Murat Department of Chemistry, Faculty of Science and Arts, Dicle University, 21280 Diyarbakır, Turkey , Çeken, Bircan Department of Chemistry, Faculty of Science and Arts, Dicle University, 21280 Diyarbakır, Turkey , Haşimi, Nesrin Department of Nutrition and Dietetics, School of Health, Batman University, 72060 Batman, Turkey , Yılmaz Özden, Tuğba fDepartment of Biochemistry, Faculty of Pharmacy, Istanbul University, 34116 Istanbul, Turkey , Demirci, Serpil Department of Pharmaceutical Botany, Faculty of Pharmacy, Cukurova University, 01330 Balcali, Adana, Turkey , Yener, İsmail Department of Analytical Chemistry, Faculty of Pharmacy, Dicle University, 21280 Diyarbakır, Turkey , Deveci, Özcan Department of Infectious diseases and Clinical Microbiology, Faculty of Medicine, Dicle University, 21280, Diyarbakır, Turkey
Abstract :
This paper is the first phytochemical and ABTS cation radical decolorisation activity,
cupric reducing antioxidant capacity, anticholinesterase and DNA damage protection effect
of endemic Verbascum pinetorum (Boiss.) O. Kuntze. Phenolic profile of V. pinetorum were
qualified and quantified by UHPLC-ESI-MS/MS analysis. Malic acid (47250.61±2504.28
μg/g) and luteolin (7651.96±527.98 μg/g) were found as most abundant compounds for metanol
and acetone extracts, respectively. Fatty acid and essential oil compositions were determined
by GC-MS analysis. The main components of fatty acid were found to be palmitic (27.1%)
and stearic (22.1%) acids. The main compounds of the essential oil were cineole (16.9%) and
α-selinene (16.4%). The acetone extract was found to be more active than BHT used as a
standard in β-carotene-linoleic acid test system. In DPPH free radical scavenging activity, the
acetone and methanol extracts showed higher activity than BHT at all tested concentrations.
The acetone, methanol and water extracts showed strong inhibition while the acetone extract
showed better activity than BHT and α-tocopherol which were used as standards in ABTS
cation radical scavenging and cupric reducing antioxidant capacity assays, respectively. All
extracts were found to be inactive in antialzheimer activity. The acetone extract exhibited
moderate antimicrobial activity against C. albicans. The methanol extract of V. pinetorum were
found no significant effect on DNA cleavage protection
Keywords :
Verbascum pinetorum , fatty acid , essential oil , antioxidant , anticholinesterase , antimicrobial , DNA cleavage protection
