Title of article
Determination of Isosorbide Dinitrate in Serum by Gas Chromatography with New Generation of Electron Capture Detector and its Application in Pharmacokinetic Study
Author/Authors
Mahmoudian, Massoud Department of Pharmacology, Iran University of Medical Sciences, Tehran , Falahatpishe, Hamidreza Pars Biopharmacy Research Laboratory, Tehran , Tayebi, Ladan Pars Biopharmacy Research Laboratory, Tehran , Gholamine, Babak Department of Pharmacology and Texicology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah
Pages
6
From page
251
To page
256
Abstract
Isosorbide dinitrate (ISDN) is an effective drug in treatment of angina pectoris. In this
study a new generation of electron capture detector (non-radioactive) with a short, non-polar
and wide-bore column was used for analysis of ISDN in human serum. ISDN was extracted
from serum by a mixture of ether and ethyl acetate and concentrated at room temperature. The
method was linear between 5-50 ng/mL. Recovery and accuracy were 99-108% and greater than
90%, respectively, and inter-day precision was lower than 13%. Pharmacokinetic parameters
were analyzed after oral administrations of Isocor 40 mg sustained release tablet in comparison
with Isoket Retard 40. The statistical results obtained from comparison of Cmax, Tmax and AUC
were reported to be bioequivalent. Further more, the method used was found to be sensitive and
accurate in pharmacokinetic studies
Keywords
Isosorbide dinitrate , Gas chromatography , Electron capture detector , Pharmacokinetic , Serum
Journal title
Astroparticle Physics
Serial Year
2009
Record number
2447307
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