Tissue Distribution of 125I-human Nonspecific Polyclonal IgG in Normal and Induced Inflammation Mice

Many different radiolabel ed antibodies have been used for radioimmunotherapy and radioimmunoscintigraphy of human diseases in animal experiments. In order to study the in vivo tissue distribution of antibody, we labeled human nonspeci fi c polyclonal IgG with Na125I using chloramine-T method. An animal model was developed by injecting turpentine in the posterior left thigh of Balb/c mice. Tissue distribution of 125I-IgG was ass essed in normal and induced inflammation mice. Although labeled IgG accumulated in normal tissues such as liver, spleen and blood, its localization in inflammatory l eft thigh was signi ficantly different from normal right thigh. Thus, by using the radiolabeling methods of IgG in order to increase the target-tobackground ratio, antibody scintigraphy could be suggested as a powerful diagnostic tool for inflammation.