Author/Authors :
Sadeghi-aliabadi, Hojjat Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences and Health services, Isfahan , Saeidi, Morteza Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences and Health services, Isfahan , Emami, Ahmad Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Mashhad University of Medical Sciences and Health services, Mashhad , Jafarian, Abbas Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Science and Health services, Isfahan
Abstract :
It has been reported that several conifers possess cytotoxic activities on some human tumor
cell lines. Taxol as a natural cytotoxic compound has been extracted from this family. In a
program to screen the cytotoxic effects of natural resources, male and female branchlets, fruit or
bark of two different species of Iranian conifers were collected, identified and the cytotoxic
effects of their hydroalcoholic extracts on three human tumor cell lines were determined.
Different concentrations of extracts were added to cultured cells and incubated for 72 h. Cell
survival was evaluated using MTT assay. Extracts from bark of female Taxus baccata showed
inhibitory activities against Hela cells. The extracts of the branchlets of male and female T.
baccata and branchlets of Cupressus horizentalis showed inhibitory activities against MDAMB-
468 cells, whereas the extracts of branchlets of female T. baccata showed inhibitory
activities against KB cells. In conclusion, extract obtained from the bark of Iranian T. baccata
showed a comparable cytotoxic effect to doxorubicin against Hela cells
Keywords :
Taxus baccata , Cupressus horizentalis , Cytotoxicity , Cancer cells
