Author/Authors :
Mostafavi Zadeh, S M Department of Microbiology - Shahed University of Medical Sciences, Tehran , Niakan, M Department of Microbiology - Shahed University of Medical Sciences, Tehran , Nedaeinia, R Department of Microbiology - Deputy of Food and Drug - Isfahan University of Medical Sciences, Isfahan - Student Research Committee - Department of Medical Biotechnology - School of Medicine - Mashhad University of Medical Sciences , Manian, M Immunology research centre - Iran University of Medical Science, Tehran , Avan, A Molecular Medicine Group - Department of Modern Sciences and Technologies - School of Medicine - Mashhad University of Medical Sciences , Nedaeinia, M Young Researchers and Elite Club - Marvdasht Branch - Islamic Azad University, Marvdasht , Ranjbar, M Department of Food Control Administration - Deputy of Food and Drug - Isfahan University of Medical Sciences , Faraji, H Student Research Committee - Department of Medical Biotechnology - School of Medicine - Mashhad University of Medical Sciences , Piroozmand, A Department of Microbiology - Kashan University of Medical Sciences, Kashan - Autoimmune Diseases Research Center - School of Medicine - Kashan University of Medical Sciences
Abstract :
Background and Aims: Cervical cancer is among leading causes of cancer related death in
women and human papilloma virus (HPV) is one of the important risk factor of this cancer.
The aim of the present study was to develop a PCR method for identification of a high
carcinogenic type of HPV, HPV 18 using E6 gene as a marker in patients with cervical
cancer
Materials and Methods: 92 Formalin-Fix (FF) and Paraffin-Embedded (PE) tissues of
premalignant and malignant lesions from cervical cancer patients were collected. DNA was
extorted followed by PCR application in two steps using L1 and E6 consensus primers.
Results: Infection with HPV was observed in 68(73.91%) out of 92 samples by L1 region
consensus primers, while 18 (26.47%) positive cases were detected to be HPV 18 using E6
type specific primer. Six of them were CINII and CINIII, and 12 cases were diagnosed as
squamous cell carcinoma.
Conclusions: Our findings demonstrated that the assessment of HPV18 using E6 gene with a
specific PCR can help in identification of high carcinogenic genotypes of HPV. Further
studies are needed to assess the value of this method in a larger multicenter setting for
establishing their values for early detection of cervical cancer patients.
