Title of article :
Sequence Analysis of the Spike Glycoprotein, Nucleocapsid Gene and 3' Untranslated Region of Razi Institute H-120 & H-52 Vaccine Strains of Infectious Bronchitis Virus
Author/Authors :
Khalesi, B Department of Research and Production of Poultry Viral Vaccine - Razi Vaccine and Serum Research institute - Agricultural Research Education and Extension Organization (AREEO), Karaj , Masoudei, Sh Department of Research and Production of Poultry Viral Vaccine - Razi Vaccine and Serum Research institute - Agricultural Research Education and Extension Organization (AREEO), Karaj , Bakhshesh, M Department of Research and Production of Poultry Viral Vaccine - Razi Vaccine and Serum Research institute - Agricultural Research Education and Extension Organization (AREEO), Karaj , Shoshtari, A H Department of Research and Production of Poultry Viral Vaccine - Razi Vaccine and Serum Research institute - Agricultural Research Education and Extension Organization (AREEO), Karaj
Abstract :
Background and Aims: Infectious bronchitis (IB) is an economically important disease of
chickens. The existence of very large number of IBV serotypes and variants which
insufficiently induce cross protection against each other is the major problem to control the
disease.
Materials and Methods: This study was performed to characterize S1, N and 3'UTR region
of the genome of H-120 and H-52 vaccine strains of Razi Vaccine Institute. The S1, N and
3'UTR region were sequenced and compared with standard strain in gene bank.
Results: Based on nucleotide identity, the S1, N and 3'UTR region of the genome of Iranian
IBV vaccine strain showed 100% similarity to the commonly standard IBV strains. To better
characterization of these strains analysis of other genes involved in virulence and
pathogenesis of the virus and performing protective tests against field strains are
recommended.
Conclusions: The results revealed that H-52 and H-120 strains of Razi institute were
identical to the standard of strains in the Gene Bank.
Keywords :
IBV , vaccine , S1 gene , nucleocapsid gene , 3' untranslated region
Journal title :
Astroparticle Physics
