Simultaneous Spectrophotometric Determination of Some Polycyclic Aromatic Hydrocarbons Using Chemometrics Methods after Their Preconcentration by SaltingOut Assisted LiquidLiquid Extraction

A rapid, powerful, satisfactory and modified signalpreprocessing method that is a hybrid of the wavelet transform with orthogonal signal correction (OSC) is used for preprocessing of spectrophotometric data of naphthalene, anthracene and pyrene as analytes after their preconcentration by saltingout assisted liquid–liquid extraction (SALLE) method. Watermiscible extraction solvent (1.0 mL of acetonitrile) and a suitable amount of salt (1.0 g of (NH4)2SO4) are added into the aqueous solution (4.0 mL) and dissolved using a vortex leading to the occurrence of phase separation. The target analytes in the sample was extracted into the watermiscible extraction solvent. After extraction, the absorbance of the analytes mixture was measured in the wavelength range of 200400 nm. The wavelet orthogonal signal correction (WOSC) was established for denoising and reduction of spectrophotometric data and hybrid with partial least squares (PLS) regression method for simultaneous determination of the studied analytes after the SALLE procedure. The influence of various parameters, such as extraction solvent and volume, type and amount of salt, vortex time and sample pH were studied and optimized. The net analyte signal (NAS) method was used for calculating figures of merit. Linear range (LR) of calibration graphs for naphthalene, anthracene and pyrene were between 0.20 2.00, 0.10 1.50 and 0.07 1.00 µg mL1, respectively. The obtained model showed good prediction capability with root mean square error of prediction (RMSEP) of 0.030, 0.024 and 0.013 µg mL1 for naphthalene, anthracene and pyrene, respectively. The simple WOSCPLS method has been successfully applied for the simultaneous determination of these analytes in the spiked wastewater samples.