Correlation between Expression of hsamiR4905p and NFAT5 in Peripheral Blood Mononuclear Cell Obtained from Breast Cancer Patients

Breast cancer is a complex genetic disease that has an average annual incidence of two million people and the second leading cause of death among women all over the world. MicroRNAs are consistently reported to regulate gene expression in all cancers. The present study, the correlation between the expression of hsamiR4905p and nuclear factor of activated Tcells 5 (NFAT5) in breast cancer were investigated. NFAT5 nuclear accumulation occurs regardless of Wnt/βcatenin activated signaling in a substantial portion of breast cancer. The analysis of prediction target and dualluciferase reporter assays supported that hsamiR4905p directly targeted NFAT5 and suppressed the expression of NFAT5. In a crosssectional comparative study, peripheral blood samples were collected from 30 subjects with breast cancer and 30 healthy individuals as a control group. Total mRNA was extracted from peripheral blood mononuclear cells (PBMCs) and cDNA was synthesized to study the NFAT5 and hsamiR4905p gene expression variations by realtime PCR. A significant decrease was observed in gene expression and sera concentration of NFAT5, hsamiR4905p in PBMCs of breast cancer patients. The obtained results indicated that hsamiR4905p acts as oncomir in serum by targeting NFAT5 direct (p lt;0.05).