Author/Authors :
Nasiri, Fatemeh Department of Biochemistry - High Institute for Research and Education in Transfusion Medicine, Tehran, Iran , Johari, Behrooz Department of Biotechnology - Pasteur Institute of Iran, Tehran, Iran , Amiri, Fatemeh Department of Biochemistry - High Institute for Research and Education in Transfusion Medicine, Tehran, Iran , Habibi Roudkenar, Mehryar Department of Biochemistry - High Institute for Research and Education in Transfusion Medicine, Tehran, Iran , Molaei, Sedigheh Department of Biochemistry - School of Medicine - Qom University of Medical Sciences, Qom, Iran , Bahadori, Marzie Department of Biochemistry - High Institute for Research and Education in Transfusion Medicine, Tehran, Iran , Movahed, Marjan Department of Biochemistry - High Institute for Research and Education in Transfusion Medicine, Tehran, Iran , Yari, Abazar Department of Anatomy, School of Medicine - Alborz University of Medical Sciences, Karaj, Iran , Jalili, Mohammad Ali Department of Biochemistry - High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract :
Introduction: Mesenchymal stem cells (MSCs) are suitable candidates for the treatment of
liver diseases. However, their low survival rate limits their efficacy following transplantation.
This study aimed to evaluate the therapeutic potentials of H2O2-preconditioned umbilical cordderived
MSCs (UCMSCs) on acute liver failure (ALF) in mice.
Methods: UCMSCs were pre-conditioned with different concentrations of H2O2. Cell viability
was evaluated by WST-1 (water soluble tetrazolium) assay followed by exposure to lethal doses
of H2O2. ALF was induced in NMRI mice using CCl4 and the cells therapy was performed
using H2O2-preconditioned and normal UCMSCs. After 24, 48, and 72 hours, regenerative
potentials of different UCMSCs groups were evaluated compared to sham group (that receive
no MSCs) using biochemical and histological methods.
Results: Lower liver enzymes was significantly evident in mice transplanted with H2O2-
preconditioned UCMSCs compared with the other groups. Interestingly, histological results
revealed a significant improvement in liver regeneration in these mice.
Conclusion: Preconditioning of UCMSCs with H2O2 not only enhances their survival but also
increases the efficacy of MSCs-based cell therapy in acute liver failure.
Keywords :
Preconditioning , Oxidative stress , Mesenchymal stem cells , Carbon tetrachloride , Acute liver failure
