Author/Authors :
Shahraki Salar, Masoud Department of Anatomy - School of Medicine - Zahedan University of Medical Sciences, Zahedan, Iran , Arab, Maryam Imam Ali Hospital - School of Medicine - Zahedan University of Medical Sciences, Zahedan, Iran , Arab, Mohamad Reza Department of Anatomy - School of Medicine - Zahedan University of Medical Sciences, Zahedan, Iran , Mashhadi, Mohamad Ali Department of Hematology and Oncology - School of Medicine - Zahedan University of Medical Sciences, Zahedan, Iran , Sargolzaei Aval, Fereydoon Department of Anatomy - School of Medicine - Zahedan University of Medical Sciences, Zahedan, Iran , Mir, Mohsen Department of Anatomy - School of Medicine - Zahedan University of Medical Sciences, Zahedan, Iran , Rigimaneshو Mohamad Department of Anatomy - School of Medicine - Zahedan University of Medical Sciences, Zahedan, Iran
Abstract :
Introduction: Cisplatin is a platinum based antineoplastic drug, which is widely used for treatment
of solid tumors. The present investigation was carried out to study the nephrotoxic effects of double
dose injection of cisplatin in rats, as an experimental model.
Methods: In this experimental study, 45 adult male Sprague Dawley rats with average weight of
200±30 g were randomly divided into two experimental (n=30) and one control (n=15) groups.
Rats of experimental groups received two repeated doses of cisplatin intraperitoneally (2.5 mg/kg,
experimental group E1 & 5 mg/kg, experimental group E2) in the beginning of first and fifth week of
the experiment. Eight weeks after injection, rats of all groups were given deep anesthesia and killed.
Blood samples were collected directly from their hearts for biochemical evaluation. Tissue samples
were removed and prepared sections were stained with H&E, PAS, Masson trichrome, and PNA
methods. Prepared microscopic slides were utilized for both histopathological and morphometrical
studies. Collected data were analyzed by ANOVA and Tukey post hoc test using SPSS.
Results: Cisplatin administration induced a significant decrease in urinary space diameter of renal
corpuscles in the experimental groups compared to the control group. This ultimately led to the
urinary space obstruction in up to 95% of nephrons in experimental groups (P<0.001). There
were significant difference between control and experimental groups (P<0.001) with regard to
epithelial thickness of collecting duct, proximal and distal convoluted tubules. Moreover, there
was a significant difference between control and experimental groups (P<0.001) with regard to
the diameter of vasa recta. Acute tubular necrosis and urinary space obstruction were the main
histological features of the experimental groups. There were also a significant elevation in serum
level of BUN and creatinine in experimental group compared to those of control group.
Conclusion: Cisplatin induces acute tubular necrosis and urinary space obstruction and some other
morphological changes in rat kidney, in a dose dependent manner.
Keywords :
Cisplatin , Convoluted tubule , Glomerulus , Vasa recta , Rat
