Evaluation of IL17 Serum Level, Brain Inflammation and Demyelination in Experimental Autoimmune Encephalomyelitis C57BL/6 Mice Model with Different Doses of Myelin Oligodendrocyte Glycoprotein

Multiple sclerosis (MS) is an autoimmune disease that affects the central nervous system.MS creates a wide range of symptoms with lifelong debilitating consequences. The hallmark of the disease is the inflammation of the nervous system, which can lead to damage to the nerve tissue and loss of function of the neurons. IL17 has a prominent role in the beginning of inflammatory reactions. Here, we analyzed a mouse model developed using antimyelin antibodies. This mouse model mimics many symptoms of MS in humans. C57BL/6 mice were randomly divided into five groups. Mice were immunized subcutaneously with 50 #x3BC;g, 100 #x3BC;g, 150 #x3BC;g and 200 #x3BC;g myelin oligodendrocyte glycoprotein in complete Freund #x2019;s adjuvant containing 4 mg/Ml Mycobacterium tuberculosis and two injections of 800 ng of pertussis toxin intraperitoneally, on day 0 and 2 post immunization. Serum level of IL17 was measured, inflammation and demyelination of brain tissue were also evaluated. Mice with experimental autoimmune encephalomyelitis demonstrated inflammatory cell accumulation, different degrees of demyelination in the brain, and rising levels of serum IL17 depending on the dose of the antimyelin antibody. #xA0;Our study demonstrates that level of IL17 production is directly associated with inflammation and demyelination. In addition, different degrees of experimental autoimmune encephalomyelitis in mice can be utilized to test a wide range of therapeutic interventions for MS treatment. #xD; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0; #xA0;