Co-Administration of Anti-Angiogenic Peptide and DNA Vaccine in Cervical Cancer Tumor Model

Background: Cervical cancer is one of the main causes of women’s death in the world. Humanpapilloma virus (HPV) types 16 and 18 have beenknownas a cause of morethan 2/3 of cervical cancers. Newcombination treatment strategies basedonimmuneresponses against viral antigens are likely to be beneficial. Considering the important role of angiogenesis in nutrition and oxygenation of tumor cells, the inhibition of this process can help tumor clearance. Objectives: In this study, co-administration of a plasmid encoding immune stimulatory epitopes of E6-E7-L1 genes of HPV with an anti-angiogenic peptide derived from Endostatin in tumor mice model was examined. Methods: C57BL/6 mice were injected subcutaneously with TC-1 tumor cells and monitored for tumor progression. At exact time points, tumor size was determined. Then they were injected by pIRES-E6/E7/L1 as DNA vaccine, and anti angiogenic peptide. Relative tumor volume measurements and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was carried out in order to investigate therapeutic antitumor effects of vaccine and peptide. Results: Based on the results, the group of mice that received vaccine and vaccine-peptide had significant inhibition rate of tumor growth in comparison with control groups (P 0.05). Lymphocyte proliferation responses against the vaccine in the related groups were significantly higher than negative control group (P 0.05). Conclusions: In general, it could be concluded that the co-administration of DNA vaccine due to the good immunogenicity and suitable inhibitory effect of peptide in reduction of tumor size, shows higher efficacy and can be considered as a new therapeutic strategy.