Survey of silver nanoparticles (AgNPs) effects on blood indices of Rainbow trout (Oncorhynchus mykiss) juveniles

Silver nanoparticles (Ag-NPs) are potentially toxicant substances whose widespread use has raised considerations regarding environmental risks caused by the discharge of those nanoparticles (NPs) into aquatic ecosystems. The aim of this study was to analyze the impact of Ag-NPs on the hematologic parameters Rainbow trout, Oncorhynchus mykiss juvenile. Hematological toxicities of Ag-NPs to rainbow trout juveniles were assessed in four treatment groups: Control (without Ag-NPs), 0.1gr/L Ag-NPs solution (T1), 0.25gr/l Ag-NPs (T2) and 0.5gr/l Ag-NPs (T3). Blood samples were collected from fish after 5 and 10 days of exposure. Analysis of blood parameters in the 5th and 10th days of the experiment showed that the values white blood cells (WBCs) were higher in all treatments than those in the control group (P 0.05) and also the red blood cells (RBCs) reduced in the high concentration of Ag-NPs in the 10th sampling day. Although the Hematocrit (Hct) and hemoglobin (Hb) concentrations were higher within the T1 and T2 groups than those in the control group both within the initial and second blood sampling, a significant decrease compared to the control group was determined in values of them both in the first and second sampling for the T3 group (P 0.05). The MCV and MCH significantly attenuated within the treatment groups within the first sampling. In contrast, the MCHC values not showed significant changes in trend because the concentration of Ag-NPs enhanced. Our results incontestable that silver nanoparticles induce important changes in hematological parameters of rainbow trout juveniles.