Author/Authors :
Kohzadi, R Department of Bioscience and Biotechnology - Malek-Ashtar University of Technology, Tehran , Molaeirad, A Department of Bioscience and Biotechnology - Malek-Ashtar University of Technology, Tehran , Alijanianzadeh, M Department of Bioscience and Biotechnology - Malek-Ashtar University of Technology, Tehran , Kamali, N Department of Bioscience and Biotechnology - Malek-Ashtar University of Technology, Tehran , Mohtashamifar, M Electronic Research Center, Tehran
Abstract :
A label-free electrochemical nucleic acid aptasensor for the detection of methamphetamine (MA) by the immobilization of thiolated
self-assembled DNA sequences on a gold nanoparticles-chitosan modified electrode is constructed. When MA was complexed specifically
to the aptamer, the configuration of the nucleic acid aptamer switched to a locked structure and the interface of the biosensor changed,
resulting in a variation of the corresponding peak current of an electrochemical probe ([Fe(CN)6]3-/4-). Two different methods of cyclic
voltammetry (CV) and microcantilever were employed to determine MA detection limit. Under the optimized experimental conditions, the
presented sensor exhibits a nice specificity towards MA. The detection limits for MA in electrode and microcantilever were obtained 10
and 0.7 nM. The proposed aptasensor can be easily regenerated by the denaturalization of aptamer-target complexes in a heated water bath
at 80-90 °C. Besides, this biosensor has a high reproducibility and selectivity, which can be a promising method to detect MA in real
samples.
