A Multidisciplinary Study to Evaluate the Anti-quorum Sensing Ability of Phyto-compounds in Ruellia patula Jacq

Background: Staphylococcus aureus (S. aureus) causing numerous diseases in humans, have become resistant to antibiotics, hence, urging the need for alternative medicines. Methods: In this study, the Indian medicinal weed, Ruellia patula (R. patula) extracted and fractioned through column chromatography was subjected to antibacterial and antiquorum sensing activity against S. aureus and Methicillin Resistant Staphylococcus aureus (MRSA). Results: The obtained results confirmed fraction F44 to have significant effect as antimicrobial and antibiofilm agent against both the micro-organism. Therefore, few of such highly active fractions were chemical finger printed using GC-MS and the compounds identified were further docked with DNA binding (LytTR) domain of agrA, which revealed that compounds identified from fraction were interactive to the pro- tein. Conclusion: R. patula is promising antimicrobial and antibiofilm agent against S. aureus and MRSA.