Pathological Evaluation of Essential Amino Acid Injection for Chicken Embryonated Eggs

Objective: The objective of this study was to investigate the pathological alterations of methionine for chicken embryo.In recent years, amino acids are used in veterinary medicine for many purposes. They injected into hatching eggs to increase chick body weight and performance at hatch, but the adverse effects of amino acids have always been a major concern. There is scant information available about the pathological alterations of amino acids in the chicken embryonated eggs. Methods: Fertile chicken eggs were divided into two equal treatment groups as follows: phosphate buffered salineinjected group and methionineinjected group whose individuals were injected with a methionine solution at a dosage of 10 mg per Kg eggweight. Embryos were reincubated posttreatment and allowed to develop until day 18 after which; they were examined for macroscopic and microscopic lesions. Results: The germination of inoculated seed was significantly reduced, germ tubes of microconidia penetrated all parts of the thick, complex seed testa, and seed contents were destroyed. Pathogenicity tests indicated that F. solani caused lesions of black soft rot and complete deterioration within 57 days (100%). They also attacked Orobanche seeds, arresting their germination and causing maceration of nongerminated and germinated seeds after 57 days of incubation. This is the first investigation of the effects of F. solanim on host plant interactions with broomrape in melon.