Isolation and identification macrocyclic spermine alkaloid(protoverbine) from verbascum speciosum

The 17-membered macrocyclic spermine alkaloid protoverbine (8-phenyl-1,5,9,13-tetra azacycloheptadecan-6-one) was isolated from the aerial part of verbascum speciosum. The natural polyamines are ubiquitous bases reported to have several important functions in animals, plants and microorganisms. They are involved in processes of transcription and translation in protein synthesis and influence growth and development (cell division, differentiation, embryogenensis, etc.). In plants, their involvement in organ development, flowering, fruit ripening, senescence and stress responses is reported. The structure of the protoverbine was established by the study of their spectral such as 1HNMR,13C-NMR, IR, ESI-MS analyses and chemical properties. To the best of our knowledge, this is the first report of protoverbine from this species.