Cloning and Expression of Recombinant Coat Protein Gene of Turnip Curly Top Virus in Escherichia Coli

Turnip curly top virus (TCTV) is a member of the newly established genus Turncurtovirus, family Geminiviridae. Development of an efficient detection system is necessary to study the biological characteristics of viruses. Due to the superior efficacy of the serological methods for detection of plant viruses, in this research, with the aim to facilitate the process of immunological detection of TCTV, coat protein (CP) gene of the virus was subcloned into prokaryotic expression vector pQE60 and transferred into E. coli strain M15. Following induction of CP expression, total protein was extracted and run onto 12.5% SDSPAGE. An approximate 34 kDa band corresponding to the TCTV CP has been expressed in the prokaryotic host. This protein could be used as antigen to induce the antibody generation for the serological detection of TCTV.