Title of article :
Molecular Characterization of Animal Fasciola spp. Isolates from Kermanshah, Western Iran
Author/Authors :
BOZORGOMID ، Arezoo - Tehran University of Medical Sciences , NAZARI ، Naser - Kermanshah University of Medical Sciences , RAHIMI ، Hamzeh - Pasteur Institute of Iran , BEIGOM KIA ، Eshrat - Tehran University of Medical Sciences , HAJJARAN ، Homa - Tehran University of Medical Sciences , MOHEBALI ، Mehdi - Tehran University of Medical Sciences , ARYAEIPOUR ، Mojgan - Tehran University of Medical Sciences , HEIDARIAN ، Peyman - Tehran University of Medical Sciences , EZATI ، Mohammad Saeid - Islamic Azad University, Science and Research Branch , ROKNI ، Mohamad Bagher - Tehran University of Medical Sciences
Abstract :
Background: We evaluated the genetic diversity of samples identified morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method. Methods: We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle, 4 goats). The species of Fasciola were confirmed by sequencing the 700 bp region of ribosomal ITS1 gene. Results: In Kermanshah, F. hepatica was present in 96% of the samples, F. gigantica was found only in two cattle sample. No hybrid forms were detected in the present study. Conclusion: Our results contribute to clarify the dark spots of Fasciola genotyping in different parts of Iran.
Keywords :
Fasciola hepatica , Fasciola gigantica , Genotype , PCR , Iran
Journal title :
Iranian Journal of Public Health
Journal title :
Iranian Journal of Public Health
