The eff ect of two Iranian viper snake; vipera albicornuta (zanjani) and vipera latifi i (lattifi i) venoms on the viability of rat bone marrow mesenchymal stem cells in vitro and in vivo

Snake venom is a complex mixture of diff erent compounds which have potential pharmacologicalproperties and may aff ect mesenchymal stem cells (MSCs). In this study we investigatethe eff ects of two Iranian vipers; vipera albicornuta and vipera latifi i crude venoms onthe viability of MScs in vivo and in vitro. Th e cells in in vitro tests were treated with diff erentconcentrations (1, 2, 3, 4 and 5 μg/100μl) of mentioned venoms for 24, 48 and 72 hours.Th e cells in in vivo experiment only were treated with v. Latifi i venom at the concentrationof 1μg/100μl and time intervals as in vitro tests. Th e cell viability in in vitro experiment wasassessed using MTT assay. Th e results of in vitro experiments showed that maximum cellviability was observed at concentrations of 1 and 2 μg/100μl of v. albicornuta and v. latifi ivenoms aft er 48 and 72 hours, respectively. Th e results of in vivo experiment showed that thecells treated with v. Latifi i venom for 72 hours in situ have the highest proliferation rate aft erpassages three, four and fi ve in comparison to control. Th e results of this study showed thatthe v. albicornuta and v. latifi i venoms can aff ect the confl uence and viability of the MSCs.