Author/Authors :
Kasalkhe ، Naghimeh Offshore Fisheries Research Center , Erfanifar ، Elnaz Offshore Fisheries Research Center , Erfanifar ، Elahe Gorgan University of Agricultural Sciences and Natural Resource , Mirzaei ، Mohammadreza Offshore Fisheries Research Center , Azini ، Mohammadreza Offshore Fisheries Research Center
Abstract :
Zinc, as one of the heavy metals and an environmental stressor, may alter many physiological processes like growth and serum parameters in fish. The main objective of this study was to determine the effects of Zinc Chloride sub-lethal concentrations (0.03 and 0.06 mg/L) on hepatic enzymes activity including enzymes, i.e. alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), in Sobaity Seabream (Sparidebtex hasta). In this study, after determining the LC50, two sub-lethal treatments of Zinc chloride include 0.03, and 0.06 mg/L were considered that any treatment had three replications. Fish were exposed to different doses of sub-lethal for 1, 5, 10 and 15 days and samples of liver tissue were taken at the end of each period. The results showed that with increasing concentration of sub-lethal levels of AST, ALP, and ALT considerably increased. In addition, with prolonged exposure amount of enzymes AST, ALP, and ALT were increased significantly (P 0.05). This investigation suggested that those hepatic enzymes as stress indicators could be used as important and sensitive biomarkers in ecotoxicological studies, concerning the effects of metal contamination and fish health.
