Antibacterial Activity of Ethyl Acetate and Methanol Extracts of Securigera securidaca, Withania sominefra, Rosmarinus officinalis and Aloe vera Plants against Important Human Pathogens

Background: Considering the importance of new antibiotics, the potential antibacterial activity of ethyl acetate and Methanol extracts of Securigera securidaca, Withania sominefra, Rosmarinus officinalis and Aloe vera plants against important human pathogens was studied. Methods: Streptococcus pneumoniae, Enterococcus faecalis, Proteus mirabilis, Staphylococcus aureus, E. coli, P. aeruginosa were the pathogenic bacteria used to determine the antibacterial effect of ethyl acetate and mathanol extracts of Securigera securidaca, Withania sominefra, Rosmarinus officinalis and Aloe vera in broth micro-dilution method. Results: The results of studying the methanol extract of S. securidaca with the lowest inhibitory concentration of 25, showed that all used bacterial pathogens were inhibited at this concentration, while the lowest inhibitory concentration of ethyl acetate extract of Securigera securidaca was 12.5, which were P. mirabilis and S. aureus is inhibited in this concentration. The highest bactericidal concentration (MBC) of S. securidaca ethyl acetate extract was 100, and S. aureus, P. aeruginosa were inhibitory at this concentration. The lowest inhibitory concentration of methanol extract of W. sominefra was 6.25, which S. pneumoniae, P. mirabilis, S. aureus and E. coli bacteria were inhibited at this concentration, while the highest inhibitory concentration was observed against P. aeruginosa (25 ppm), The lowest inhibitory concentration of the ethyl acetate W. sominefra extract was 3.1ppm in comparison with P. mirabilis and E. coli. Conclusion: In conclusion, it seems that S. securidaca, W. sominefra, R. officinalis and Aloe vera extracts could inhibit the growth of all of the mentioned bacteria.