Author/Authors :
Bassiri ، Farzaneh - Islamic Azad University, Fars Science and Research Branch , Nasr-Esfahani ، Mohammad Hossein - Academic Center for Education, Culture and Research (ACECR) , Forozanfar ، Mohsen - Islamic Azad University, Fars Science and Research Branch , Tavalaee ، Marziyeh - Academic Center for Education, Culture and Research (ACECR)
Abstract :
Background:Imbalance between production of reactive oxygen species (ROS) and total antioxidant capacity in testis, epididymis, and seminal fluid can eventually lead to infertility. Abnormal sperm chromatin packaging, and DNA fragmentation is considered as the main underlying causes of infertility. Therefore, we aimed to assess relationship between sperm parameters with DNA damage, protamine deficiency, persistent histones, and lipid peroxidation in infertile men with at least one failed cycle after intracytoplasmic sperm injection (ICSI). Materials and MethodsIn this experimental study, semen samples were collected from infertile men with at least one failed intracytoplasmic sperm injection (ICSI) cycle (n=20). Sperm parameters, DNA damage, protamine de ficiency, persistent histones, and lipid peroxidation were assessed using computerassisted sperm analysis (CASA) system, sperm chromatin structure assay (SCSA) and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays, chromomycin A3, aniline blue, and BODIPY C11 staining, respectively.Results:A negative significant correlation was observed between sperm concentration with percentage of sperm persistent histones (r=0.56, P=0.02), while positive significant correlations were found between percentage of sperm persistent histones with percentage of abnormal morphology (r=0.54, P=0.02), CMA3positive spermatozoa (r=o.6, P=0.008) and intensity of lipid peroxidation (r=0.6, P=0.01). In addition, significant correlation was observed be tween sperm DNA damage with intensity and percentage of lipid peroxidation (r=0.62, P=0.009). Correlation between CMA3positive spermatozoa and intensity of lipid peroxidation (r=0.5, P=0.03) were also significant.Conclusion:Observed significant correlations between sperm functional tests in infertile men with at least one failed ICSI cycle, indicated that the reduction of oxidative stress by antioxidant supplementation may be considered as one therapy approach that can improve sperm function and increase the chance of successful clinical outcomes in next assisted reproductive cycle.
Keywords :
DNA Damage , Intracytoplasmic Sperm Injections Spermatozoa , Lipid Peroxidation , Protamines ,
