Title of article :
Oropharyngeal Colonization of Haemophilus influenzae Type b and Serologic Response After Administration of Third Dose of Pentavalent Vaccine to 12-Month-Old Children in Karaj, Iran, 2016
Author/Authors :
Shirvani, Fariba Pediatric Infections Research Center - Research Institute for Children Health - Shahid Beheshti University of Medical Sciences, Tehran , Arjmand, Reza Department of Pediatric - Emam Ali Hospital - Alborz University of Medical Sciences, Karaj , Golami, Mehri Alborz University of Medical Sciences, Karaj , Najafi Tavana, Esfandiar Department of Pediatric - Emam Ali Hospital - Alborz University of Medical Sciences, Karaj , Saee Rad, Samira Medical Genetic Laboratory, Karaj , Pourrostami, Kumars Department of Pediatric - Emam Ali Hospital - Alborz University of Medical Sciences, Karaj , Safari, Omid Department of Pediatric - Emam Ali Hospital - Alborz University of Medical Sciences, Karaj , Nikkhah, Saeed Department of Pediatric - Emam Ali Hospital - Alborz University of Medical Sciences, Karaj , Qorbani, Mostafa Endocrinology and Metabolism Research Center - Endocrinology and Metabolism Clinical Sciences Institute - Tehran University of Medical Sciences, Tehran , Elahimehr, Nasrin Department of Pediatric - Emam Ali Hospital - Alborz University of Medical Sciences, Karaj , Zahmatkesh, Ehsan Department of Pediatric - Emam Ali Hospital - Alborz University of Medical Sciences, Karaj , Seifi, Kimia Young Researchers and Elite Club - Roudehen Branch - Islamic Azad University, Roudehen
Abstract :
Background: The administration of Haemophilus influenzae type b (Hib) conjugate vaccine led to a decrease of over 90% in the
prevalence of severe Hib diseases in the countries with universal coverage vaccine. After addition of Hib vaccine to the national
vaccination program and since no study has yet investigated this subject.
Objectives: The current study aimed at investigating the serologic response and assessing oropharyngeal colonization with Hib
after the last dose of vaccine.
Methods: A total of 500 blood and oropharyngeal samples were collected from one-year-old children referred to Karaj health care
centers, Iran. Demographic information and risk factors of the children were collected. Oropharyngeal and blood samples were
transferred to the laboratory to determine antibody titer by the enzyme-linked immunosorbent assay (ELISA) technique, culture
testing, and polymerase chain reaction (PCR).
Results: In the current study, 11.8% of children (95% confidence interval (CI): 8.97 - 14.63) had an anti-Hib IgG titer of 5 g/mL.
Geometric mean titer (GMT) of vaccine antibody was 6.92 g/mL (95% CI: 6.76 - 7.08); 9% of oropharyngeal culture results were
positive for H. influaenzae (non-type b) and 8.2% were confirmed by PCR. Prevalence of oropheryngeal Hib colonization was 0.02%.
There was no significant correlation between the titer of H. influaenzae antibody and positive culture of H. influaenzae and the other
studied variables (P > 0.05).
Conclusions: In Iran, similar to most countries, pentavalent vaccine in national vaccination program decreased the prevalence of
Hib colonization. Prevalence of Hib colonization is an important factor in invasive diseases incidence. It is suggested that further
studies asses the prevalence of invasive Hib diseases after national vaccination.
