Title of article
Molecular Identification of Gram-Negative Bacteria in Respiratory Samples of Cystic Fibrosis Patients from a Children Referral Hospital in Tehran
Author/Authors
Azimi, Leila Pediatric Infections Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran , Mansour Ghanaiee, Roxana Pediatric Infections Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran , Shirdust, Mehdi Pediatric Infections Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran , Moein Karimi, Ahmad Tehran Medical Science Branch - Islamic Azad University, Tehran , Armin, Shahnaz Pediatric Infections Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran , Rafiei Tabatabaei, Sedigheh Pediatric Infections Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran , Shiva, Farideh Pediatric Infections Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran , Fallah, Fatemeh Pediatric Infections Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran , Rezaei, Mohammad Alborz University of Medical Sciences, Alborz , Fahimzad, Alireza Pediatric Infections Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran , Khanbabaei, Ghamartaj Pediatric Infections Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran , Rahbar, Mohammad Pediatric Infections Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran
Pages
5
From page
1
To page
5
Abstract
Background: Gram-negative bacteria are a major cause of pulmonary infection in patients with cystic fibrosis (CF).
Objectives: The study aimed to conduct the molecular identification of Gram-negative bacterial flora causing pulmonary infection
in children with CF.
Methods: In this study, sputum samples were taken from 64 CF children undergoing treatment as outpatients or inpatients at a
referral children’s hospital in Tehran. The PCR technique was used to detect the presence of Gram-negative bacteria, namely Pseudomonas
spp., Acinetobacter baumannii, Stenotrophomonas maltophilia, and Burkholderia cepacia.
Results: All samples were positive for 16srRNA. Pseudomonas spp. and A. baumannii were detected in 47% and 14% of the studied
samples, respectively. Co-colonization by Pseudomonas spp. and A. baumannii was observed in three (5%) samples.
Conclusions: According to this survey, Pseudomonas spp. were the most prevalent Gram-negative bacteria isolated from CF patients
with pulmonary infection by molecular assays.
Keywords
Pseudomonas , Cystic Fibrosis , Polymerase Chain Reaction
Journal title
Astroparticle Physics
Serial Year
2019
Record number
2469788
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