Effect of Temperature of Water Used For Reconstitution On Stability Of Antibiotic Dry Suspension

This study is carried out to test the stability of antibiotic dry suspensions reconstituted by various water temperatures. The testing included physical, microbial and chemical changes of samples reconstituted by water at 40, 60, 70 and 80°C. These changes were compared to control samples prepared by water at temperature 25°C. HPLC method was followed qualitatively to identify antibiotic active constituent in addition to quantitative analysis to evaluate antibiotic contents as compared to the control samples. The changes were assessed within one hour after reconstitution and after four days of reconstitution. Physical tests showed changes of amoxicillin\clavulenic acid suspension’s colourprepared at 80ᵒC. Sedimentation ratio, sedimentation rate and sedimentation volume decreased as temperature increased. Besides, these parameters were tested only for amoxicillin suspension.Upon centrifugation, there was a decrease in sediment volume accompanied by an increase in supernatant volume resulting in changes in sediment/supernatant ratio. Microbial study showed a marked decrease in antimicrobial activity for both amoxicillin and amoxicillin/clavulenic acid suspensions. HPLC results showed a decrease in amoxicillin and clavulanate in samples prepared by heated water as compared to those prepared by cooled water at 25°C.