Inhibitory Effect of Nanogel/Emulsion of Chitosan Coating Incorporated with Ziziphora Clinopodioides Essential Oil and Nisin on Escherichia Coli O157:H7 Inoculated in Beef at Cold Storage Condition

Introduction: This study was conducted to evaluate the influence of chitosan Nanogel/emulsion coating functionalized by Ziziphora clinopodioides essential oil (ZCEO) and nisin on growth inhibition of Escherichia coli O157: H7 inoculated in beef samples during 16 days in cold storage condition (4˚C). Methods: Beef sample was divided into six groups after inoculation of E. coli O157:H7. Treatments including control (no coating), chitosan 2%, sonicated chitosan 2%, Nanoemulsion of chitosan coating containing ZCEO (0.5%), Nanogel of chitosan coating containing nisin (200 IU/g), Nanoemulsion of chitosan coating containingZCEO (0.5%) and nisin (200 IU/g) were stored at refrigeration temperature and bacterial count were performed on days: 0, 1, 2, 4, 8, 12 and 16. Data were analyzed using repeated measure ANOVA and Bonferroni post hoc tests. Results: Result indicated a significant reduction in E. coli O157:H7 count in all treatments when compared to control group and the highest inhibitory activity was observed in chitosan Nanoemulsion coating containing ZCEO (0.5%) and nisin (200 IU/g). Conclusion: Accordingly, it is suggested that chitosan Nanoemulsion coating with ZCEO and nisinpractically be applied in beef to increase its safety against pathogenic bacteria especially E. coli O157: H7.