Author/Authors :
Zarei Mahmoudabadi, Ali School of Biological Sciences - University of Manchester - Manchester - UK , Drucker, David Bernard Turner Dental School - University of Manchester - Manchester - UK , Mandall, Nicky Turner Dental School - University of Manchester - Manchester - UK , O’Brien, Kevin Turner Dental School - University of Manchester - Manchester - UK , Theaker, Elizabeth Turner Dental School - University of Manchester - Manchester - UK
Abstract :
CHROMagar Candida is a new chromogenic culture medium that is used for the isolation and direct
identification of some of the most clinically important yeast pathogens on the basis of colony colour.
The aim of the study was to isolate and identify Candida species from the oral cavity with
CHROMagar Candida. Samples (158) from 68 orthodontic patients wearing upper removable
orthodontic appliances and 51 oral medicine patients were cultured on CHROMagar Candida at 37C
for 48 h. Isolates with green colonies in colour were all confirmed as C. albicans (53) by germ tube test
and others were confirmed as C. parapsilosis (10) and C. glabrata (3) by Rapid ID 32 C kits. There
were 11 cases of mixed yeast cultures in this study that were easily recognised using the medium. In
conclusion, C. albicans is the most frequently isolated yeast from the oral cavity of both orthodontic
and oral medicine patients. Moreover CHROMagar Candida is a useful culture medium for the
isolation and direct identification of Candida species, especially mixed cultures. I
