Title of article
Detection of Toxoplasma Gondii and Human Cytomegalovirus DNA in Blood from Transplant Recipients Using Multiplex Nested Polymerase Chain Reaction
Author/Authors
Khodai Booran, Nasser Pasteur Institute of Iran , Assmar, Mehdi Pasteur Institute of Iran , Hovanessian, A Pasteur Institute of Iran , Nikolaova, N lnstitute of Molecular Genetics - Moscow - Russia , Bakayevl, Valery V Pasteur Institute of Iran
Pages
5
From page
89
To page
93
Abstract
Evidences from many studies suggested a polymerase chain reaction (PCR) as a valuable method for
diagnosing infectious disease in the transplant recipients. We used this method for detection of
Toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or
kidney transplantation. DNA of both infectious agents were detected using two separate sets of nested
primers in the PCR. The conditions for a multiplex nested PCR providing simultaneous identification of
both pathogens in one tube were optimized. This assay provides an application in clinical research for
diagnosis of infections in post-transplant recipients.
Keywords
Toxoplasma Gondii , HCMV , Multiplex PCR
Journal title
Astroparticle Physics
Serial Year
1998
Record number
2474297
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