Author/Authors :
Khodai Booran, Nasser Pasteur Institute of Iran , Assmar, Mehdi Pasteur Institute of Iran , Hovanessian, A Pasteur Institute of Iran , Nikolaova, N lnstitute of Molecular Genetics - Moscow - Russia , Bakayevl, Valery V Pasteur Institute of Iran
Abstract :
Evidences from many studies suggested a polymerase chain reaction (PCR) as a valuable method for
diagnosing infectious disease in the transplant recipients. We used this method for detection of
Toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or
kidney transplantation. DNA of both infectious agents were detected using two separate sets of nested
primers in the PCR. The conditions for a multiplex nested PCR providing simultaneous identification of
both pathogens in one tube were optimized. This assay provides an application in clinical research for
diagnosis of infections in post-transplant recipients.
